Bizhan Mahmoudi; Hedayatollah Roshanfekr; Mohsen Sari; Mohammad Reza Bakhtiarizadeh
Volume 22, Issue 3 , September 2020, , Pages 337-348
Abstract
The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine ...
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The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine platform. Hisat2 software was used to align reads to the bovine reference genome and StringTie software package was used toassemble read files into transcripts. Using next generation sequencing, 1636 genes belonging to known intergenic lncRNAs were identified, of which56 genes showed significant differential expression (P≤0.05). Neighbor genes of known intergenic lncRNAs were determined on bovine genome.Analysis of biological pathways and molecular function showed that five biological pathways were significantly (P≤0.05) enriched. These pathwayswere Apelin signaling pathway, Gap junction, Glucagon signaling pathway, Renin secretion, and AGE-RAGE signaling pathway. Moreover, twomolecular functions including gap junction channel activity, and phosphatidyl inositol phospholipase C activity were significantly (P≤0.05) enriched.Some lncRNAs have different expression in healthy and acidosis samples, and the decreased pH acts as a stimulus to activate some biologicalsignaling pathways. In conclusion, it was indicated that lncRNAs with differential expression between the control group and the group affected byacidosis are associated with pathways related to rumen energy metabolism and signaling. Identified differentially expressed lncRNAs could be used asprognostic in acidosis and biomarkers or promising candidates in animal breeding.
Seyyed Majid Azarm; Somayyeh Salari; Mohsen Sari; Mohammad Reza Ghorbani; Mohammad Hojjati
Volume 22, Issue 2 , June 2020, , Pages 301-312
Abstract
This experiment was conducted to evaluate the effect of Thyme essential oil on performance and some physiological parameters of broiler chickens fed diets containing wheat in a completely randomized design with a 2×3 factorial arrangement using 264 broilers (Ross 308) in ground floor. Treatments ...
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This experiment was conducted to evaluate the effect of Thyme essential oil on performance and some physiological parameters of broiler chickens fed diets containing wheat in a completely randomized design with a 2×3 factorial arrangement using 264 broilers (Ross 308) in ground floor. Treatments consisted of 2 levels of wheat (0 and 50% of the diet) and 3 levels of essential oil of Thyme (0, 200 and 400 ppm). Performance parameters were recorded weekly. Determination of micro-flora, carcass characteristics, and blood parameters were done on day 42. The results showed that adding the Thyme essential oil to the diet containing 50% wheat increased feed intake at the all period of experiment. Body weight gain was increased significantly in the birds that consumed 50% wheat with 400 ppm essence compared to the birds consumed the diet without wheat or without essence at the starter period. The birds consumed the diet without wheat with essence had lower blood concentration of LDL. Although, adding essence at the level of 400 ppm to the diet without wheat increased significantly blood concentration of HDL. The diet contained 50% wheat without essence had higher abdominal fat compared to the diet with 200 and 400 ppm of essence without wheat and also the diet contained 50% wheat with 400 ppm essence. Overall, the use of Thyme essence at the level of 400 ppm in wheat-based diet is recommended for increasing feed intake and improving some physiological parameters of broiler chickens.
sadegh asadollahi
Volume 19, Issue 1 , May 2017, , Pages 99-115
Abstract
The effect of partial replacement of barley with sugar beet pulp (SBP) with and without roasted canola seeds (RCS) on the performance, morphology, VFA and digesta pH in the small intestine of finishing lambs was investigated. In this study 24 Arbia male lambs with average body weight of 23.7±2.5 ...
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The effect of partial replacement of barley with sugar beet pulp (SBP) with and without roasted canola seeds (RCS) on the performance, morphology, VFA and digesta pH in the small intestine of finishing lambs was investigated. In this study 24 Arbia male lambs with average body weight of 23.7±2.5 kg were used in a 2 × 2 factorial experiment over 84 days. Treatments were carbohydrate factor (barley or SBP) and roasted canola seed (with and without RCS). Partial replacement of barley with SBP significantly increased average daily gain (P < 0.05). Partial replacement of barley grain with SBP increased thickness of muscle, crypt depth and Brunner’s glands in duodenum and villus height, villous width, villus number, thickness of the epithelium, thickness of muscle, crypt depth and villous height to crypt depth in jejunum and villus height and thickness of muscle in ileum (p<0.05). Feeding lambs with diets containing RCS significantly increased Brunner’s glands,villus number and the thickness of the epithelium (P<0.05). Partial replacement of barely by SBP and adding RCS to diet increased significantly concentration individual VFA, total VFA and digesta pH in duodenum and jejunum. Based on the results, the performance of lambs improved as a result of partial replacement of barley with SBP and addition RCS to diets with high concentrate due to changes in the density of volatile fatty acids, digestive fluid pH of the small intestine and the development of the small intestine tissue morphology.
Abbas Rajaei Rad; Mohsen Sari; Mohammad javad Zamiri; Morteza Chaji; Somayeh Salari
Volume 18, Issue 3 , October 2016, , Pages 431-445
Abstract
Palm date leaves were processed with lime in a processing reactor in a factorial arrangement of treatments (3 × 3) with 3 levels of temperatures (40, 80 and 100°C) and time periods (80, 160, and 240 minutes) in a completely randomized design. Fermentation parameters measured using gas production ...
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Palm date leaves were processed with lime in a processing reactor in a factorial arrangement of treatments (3 × 3) with 3 levels of temperatures (40, 80 and 100°C) and time periods (80, 160, and 240 minutes) in a completely randomized design. Fermentation parameters measured using gas production technique. With increasing pretreatment time and temperature lignin content reduced from 13.0 percent in untreated leaves to 6.0 percent in leaves treated in 100°C for 240 min. Increasing time and temperature of the processing decreased ash content (P<0.01). Calcium concentration was increased from 0.58 percent in untreated leaves to 0.94 in the processed biomass at a temperature of 40°C for 80 min (P<0.01). Interaction was significant among pretreatment time and temperature on cell wall, dry matter loss, gas production from fermentable fraction and gas production (P<0.01). Gas production at the end of 72 h incubation increased from 76.2 ml per gram of dry matter in untreated samples to 189.8 ml per gram of dry matter in the treatment of 100°C for 240 min. Organic matter digestibility increased with increasing severity of pretreatment from 26.5 percent in the treatment of 40°C for 80 min to 36.4 percent in the treatment of 100°C for 240 min. The images of scanning electron microscopy showed more pores and holes in pretreated samples with increasing temperature and processing time compared to untreated sample. Results indicated that lime pretreatment with heat can remove lignin and improve in vitro digestibility of date leaves.
D Shafieipour fard1; Somayeh Salari; Mohsen Sari; Saman Abdanan Mehdizadeh; Mehdi Zarei
Volume 18, Issue 3 , October 2016, , Pages 539-552
Abstract
This experiment was conducted to evaluate the effect of different lipid sources and organic zinc supplementation on performance, egg bacterial activity and ovary morphology of laying hens for 10 weeks. with 288 sixty weeks old birds by employing a completely randomized design with factorial arrangement ...
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This experiment was conducted to evaluate the effect of different lipid sources and organic zinc supplementation on performance, egg bacterial activity and ovary morphology of laying hens for 10 weeks. with 288 sixty weeks old birds by employing a completely randomized design with factorial arrangement of 3×4 with 12 treatments, 4 replicates and 6 hens each. The factors of interest were 4% lipid from different sources (soybean oil, fish oil and tallow) and various levels of zinc (40, 100, 200 and 300 mg/kg of diet). In all three lipid sources, significant reduction (P<0.05) was observed in feed intake when the level of zinc supplementation increased. Haugh unit was significantly (P<0.05) increased in diets containing different lipid sources as the level of zinc supplementation increased. The highest Haugh unit value was observed at the level of 100 mg/kg of zinc supplementation with soybean oil and fish oil. Increasing levels of zinc supplementation significantly (P<0.05) improved eggshell thichness, eggshell strength and eggshell percentage. The number of large white follicles linearly increased in diets containing tallow, when the level of zinc supplementation increased. The egg yolk microbial load was significantly (P<0.05) decreased by different lipid sources as the level of zinc supplementation increased. According to the results, it seems that the addition of zinc to the diets containing different lipid sources could not affected performance parameters but decreased egg yolk microbial load.
Zeinab Moradpour; Somayeh Salari; Mohammad Reza Ghorbani; Mohsen Sari
Volume 18, Issue 2 , June 2016, , Pages 261-272
Abstract
This experiment conducted to evaluate the effect of different methods of force molting on performance, egg quality of laying hens with one hundred eighty of 52-wk old Hy-line layer in completely randomized design with six treatments, five replicates for 90 days. Treatments include 1 - feed withdrawal ...
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This experiment conducted to evaluate the effect of different methods of force molting on performance, egg quality of laying hens with one hundred eighty of 52-wk old Hy-line layer in completely randomized design with six treatments, five replicates for 90 days. Treatments include 1 - feed withdrawal (FW), 2 - 50 percent alfalfa meal: 50 percent layer ration, 3 - 75 percent alfalfa meal: 25 percent layer ration, 4 - 100 percent alfalfa meal, 5 - whole barley grain (WBG) and 6 - high level of zinc oxide (20000 ppm) that were used for 10 days. The highest percentage of weight loss was observed in FW and zinc oxide groups (P<0.05). Egg production of birds that fed 50 percent alfalfa meal: 50 percent layer ration stopped later and reached to 50 percent egg production sooner than the other treatments (P<0/05). The WBG and 100 percent alfalfa meal had better egg production feed conversion ratio in compare to FW birds (P<0.05). The mean egg weight observed higher in FW group and egg yolk color was higher in FW and WBG groups in compare to the other treatments (P<0.05). The WBG and zinc oxide groups had highest eggshell percentage (P<0.05). According to the results of this experiment it seems that WBG and 100 percent alfalfa meal groups are the useful methods for force molting because of improving performance parameters of layers.
Morteza Nadali; Somayyeh Salari; Mohammad Boujarpour; Saleh Tabatabaei Vakili; Mohsen Sari
Volume 17, Issue 1 , April 2015, , Pages 141-149
Abstract
Effect of levels of zinc supplementation on footpad lesions, reproductive and blood parameters of broiler breeders with 300 hens and 40 roosters of Ross 308 strain with four treatments, five replicates and 15 hens and two roosters in each for 12 weeks was evaluated in a completely randomized design. ...
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Effect of levels of zinc supplementation on footpad lesions, reproductive and blood parameters of broiler breeders with 300 hens and 40 roosters of Ross 308 strain with four treatments, five replicates and 15 hens and two roosters in each for 12 weeks was evaluated in a completely randomized design. Dietary treatments were 80, 110, 140 and 170 mg zinc/kg diet. Increasing supplemental zinc level especial 170 mg/kg diet increased hatchability (P<0.05). Levels of 140 and 170 mg zinc/kg diet significantly improved footpad lesions (P<0.05). In the first phase of incubation, the lowest rate of mortality observed in 140mg zinc/kg diet (P<0.05). Also, number of large and small size follicles of broiler breeders increased at 170 mg zinc/kg diet (P<0.05). Blood parameters did not affect significantly. The results of this experiment showed that supplementation of broiler breeders diet with zinc at 140 and 170 mg/kg diet could improve hatchability and had desirable effect on footpad health.
Rohollah Ebrahimi; Tahereh Mohammadabadi; Mohsen Sari; Somayeh Sallari; Mohammadjavad Zamiri; Mohammadtaghi Beigi Nassiri
Volume 15, Issue 2 , October 2013, , Pages 127-138
Abstract
In order to investigate the effect of different levels of silymarin (0, 100 and 200 mg/kg diet) onperformance, carcass characteristics, blood parameters and antioxidant status, 120 day-old chicks wereassigned to 3 treatments with four replicates and 10 birds in each in a completely randomized design.Results ...
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In order to investigate the effect of different levels of silymarin (0, 100 and 200 mg/kg diet) onperformance, carcass characteristics, blood parameters and antioxidant status, 120 day-old chicks wereassigned to 3 treatments with four replicates and 10 birds in each in a completely randomized design.Results showed supplementation of 200 mg silymarin significantly decreased the body weight gain(BWG) in starter (P<0.05) and whole period of experiment (P<0.01) and significantly increased feedconversion ratio at starter (P<0.05), grower (P<0.05) and total rearing periods (P<0.01). Also, addition of100 mg silymarin significantly increased the carcass relative weight (P<0.01) and supplementation of 200mg silymarin significantly increased the breast relative weight (P<0.05). Furthermore, 200 mg silymarinsignificantly increased the malondialdehyde and heterophil/ lymphocyte ratio and significantly decreasedsuperoxide dismutase (P<0.05). These results showed that supplementation of silymarin in diet undernormal condition, improved the carcass and breast relative weight, but no had effect on bird’s oxidativestatus.